front of the jury composed of:
– Ms Andréa Pasc, Professor, University of Lorraine, Nancy- Rapporteur
– Mr Cédric Mayer, Professor, Université Paris-Saclay, Paris – Rapporteur
– Mr Suming Li, Research Director, CNRS, Montpellier – Examiner
– Ms Hélène Greige-Gerges, Professor, Lebanese University, Lebanon – Examiner
– Mr Houssam Rassy, Professor, American University of Beirut, Lebanon – Examiner
– Mr Mihail Barboiu, Research Director, CNRS, Montpellier – French PhD supervisor
– Mrs Gihane Nasr, Professor, Lebanese University, Lebanon – Lebanese PHD superisor
The original exploratory research carried out for this report consists of a novel design of functional and hybrid nanomaterials assembled through supramolecular interactions and dynamic covalent chemistry. Poly(amidoamine) (PAMAM) dendrimers interact with liposomes through supramolecular interactions to form hybrid nanosystems. The presence of G4 PAMAM increased the encapsulation efficiency of p-CA compared to single liposomes and slowed down its release. Therefore, our attempt to combine dendrimer and liposome seemed to be beneficial to encapsulate low-water soluble molecules. PEGylated dynamic constitutional frameworks (DCFs) were synthetized via aldehyde/amino_imine chemistry. The implementation of DCFs within liposomes or gold nanoparticles to generate cross-linked networks that can self-fabricate, adapt, and respond, in a controlled fashion is unprecedented. Advances in the synthesis of liposomes, gold nanoparticles (AuNps) and functional DCFs were reported, differing in the concentration and composition. The nanometric and spherical morphology of DCFs/liposomes hybrid networks support their use as carrier devices for p-CA. They increased significantly the solubility of p-CA due to its physical entrapment additionally to possible hydrophobic and electrostatic interactions. Furthermore, the release of p-CA was sustained during time. We synthesized double cross-linked Au-DCFs networks with multiple integrated functions. Several strategies were elaborated and optimized to ensure best immobilization of carbonic anhydrase that catalyze the reversible hydration of carbon dioxide. High binding affinity and activation effects were demonstrated within DCFs and Au-DCFs networks. To conclude, we developed hybrid networks as intelligent materials that are self-fitted by adaptation to the environment. These features output their performance in drug delivery and enzyme activation compared to corresponding static and single systems.
Keywords: dynamic constitutional chemistry – liposomes – PAMAM dendrimers – gold nanoparticles – dynamic constitutional networks – para-coumaric acid – carbonic anhydrase activation.